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Pyrimidine based Receptor for Advanced Glycation Endproducts (RAGE) inhibitors as an anti-inflammatory compounds

The interaction between RAGE and its ligands is thought to result in pro-infl ammatory gene activation. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-κB). NF-κB controls several genes which are involved in infl ammation. Interestingly, RAGE itself will also be up-regulated by NF-κB. Given a condition in which there is a large amount of RAGE ligands (e.g. AGE in diabetes or Amyloid- β-protein in Alzheimer’s Disease) this establishes a positive feed-back cycle, which leads to chronic infl ammation. This chronic condition is then believed to alter the micro- and macrovasculature in a fatal way which ends in organ damage or even organ failure. Diseases that have been linked to RAGE are: atherosclerosis, peripheral vascular disease, myocardial infarction, congestive heart failure, diabetic retinopathy, diabetic neuropathy, diabetic nephropathy, Alzheimer’s disease, psoriasis.

Receptor for advanced glycation endproducts plays an important role in many pathological processes and thus represents potential for research and development of diagnostic and therapeutic strategies. Although there were several small molecular RAGE inhibitors described in the literature, they mostly do not showed proof-of-concept therapeutic potential, and thus, there is still potential for future developments in the field.

We have discovered a group of pyrimidine derivatives with proprietary structure and ability to inhibit RAGE receptor (low micromolar concentrations). The effects were described under in vitro conditions using RAGE ligand (β-amyloid, S-100 protein and glycated albumin) dependent activation of NFκB, MAPK/JNK (AP-1) and JAK/STAT (STATJ) signaling. The binding was further confi rmed and SAR elucidated by docking against the crystal structure of the RAGE. In vitro validation experiments have also demonstrated inhibition of RAGE ligand dependent activation of NO production in macrophages cells co-stimulated with IFN-γ. The compounds have reasonable toxicity, regiments with 50 mg/kg (2-3x daily) orally were well tolerated in rat model of rheumatoid arthritis and infl ammatory bowel diseases (IBDs). The model compound was signifi cantly active in IBD (comparable effi cacy to sulfasalazine), but not in RA, which seems to be in agreement with published data on RAGE role in IBD versus RA.

Laboratory scale, data on cell lines, structural data from receptor docking, limited ADME/Tox data, in vivo pharmacology and pharmacodynamics.

Patent protection: Patent protection is scheduled for 2016. For more details a CDA/NDA is required.

Palacky University Olomouc - Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences Institute of Experimental Medicine, Czech Academy of Sciences