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Light source with uniform energy density for in vitro induction of photodynamic effect in cells

INTRODUCTION: 
Photodynamic therapy (PDT) is besides chemotherapy, radiotherapy and immunotherapy other option for treatment of tumor diseases. It is a kind of photochemotherapy that is characterized by a harmful effect of photodynamically active drug and light in the presence of oxygen molecules. The therapeutic principle is based on whole-body or local administration of the sensitizers followed by UVA or VIS irradiation. Light emitting diodes (LEDs) nowadays more often are used as a source of radiation. The effi ciency of PDT signifi cantly depends on the type of sensitizer, its concentration and irradiation dose. Therefore, we have developed a source with an uniform arrangement of LEDs to homogenize such irradiation.

TECHNOLOGY (INVENTION) DESCRIPTION: 
Light source with uniform energy density for in vitro induction of photodynamic effect in cells is assembled from a fi eld of neighboring superfi cially emitting LED diodes arranged into hexagonal form, where triplet of neighboring LED diodes forms equilateral triangle. LED diodes are fi xed to a board opposite sample place arranged on distance elements, where six neighboring LED diodes is everywhere arranged in constant distance from randomly selected LED diodes lying out of the fi eld edge.

ADVANTAGES OVER EXISTING SOLUTIONS: 
Light source with uniform energy density for in vitro induction of photodynamic effect in cells comprises a fi eld of neighboring superfi cially emitting LED diodes, where the distance between 2 individual diodes is constant from 1 to 9 mm. Led diodes fi xed into a pad are exchangeable. The light fi eld is height adjustable. The source comprises a controlled cooling device.

DEVELOPMENT STATUS (STAGE): 
Prototype.

PUBLICATIONS: 
Krestyn, E., Kolarova, H., Bajgar, R., Tomankova, K. Photodynamic properties of ZnTPPS4, ClAlPcS2 and ALA in human melanoma G361 cells. Toxicology in Vitro, Volume 24, Issue 1, February 2010, Pages 286-291 Kolarova, H., Nevrelova, P., Bajgar, R., Jirova, D., Kejlova, K., Strnad, M. In vitro photodynamic therapy on melanoma cell lines with phthalocyanine. Toxicology in Vitro, Volume 21, Issue 2, March 2007, Pages 249-253 Kolarova, H., Bajgar, R., Tomankova, K., Nevrelova, P., Mosinger, J. Comparison of sensitizers by detecting reactive oxygen species after photodynamic reaction in vitro. Toxicology in Vitro, Volume 21, Issue 7, October 2007, Pages 1287-1291

IP PROTECTION STATUS: 
Patents: CZ 302829

TECHNOLOGY / IP OWNERS : 
Palacky University Olomouc - Institution of Molecular and Translational Medicine (IMTM), Faculty of Medicine and Dentistry.